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GrainGenes Probe Report: WMC23

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC23
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2a9 Donor: Wang, Utah Big insert with 2 large CT microsatellites. Numerous small motifs(CT)22 55 to 98, (CT)18 853 to 888. Primers flank 1 microsatellite only. Product of right size , maybe polymophic but too large and smeary to be sure. Reduce size of fragment? Bases 852 to 867 show homology to 22d11 bases 118 to 133 (16 in longest run, maxScore = 20).
Type	PCR SSR
PCR primers	Forward ATTcgcTcATAcgATAgggTTg at 11 length 22 bases, Tm 62.0 Reverse AgAggcTggTgTAgTTggTTTg at 303 length 22 bases, Tm 62.0
SSR size	314 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC23
Source Species	Triticum aestivum