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GrainGenes Probe Report: WMC21

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC21
Locus	wmc21 wmc21-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2g7 Donor: Wang, Utah (GA)37 29 to 102. Faint smeary PCR product after amplification at 61C anneal. Matches 3e6.
Type	PCR SSR
PCR primers	Forward cgcTgccgTgTAAcTcAAAATc at 6 length 22 bases, Tm 66.0 Reverse AgTTAATTgggcgcTccAAgAA at 120 length 22 bases, Tm 66.0
SSR size	136 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC21
Source Species	Triticum aestivum