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GrainGenes Probe Report: WMC20

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC20
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1G6 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 119 bases located on 1A AGR47. CA microsatellite. Trimmed to Rsa1 fragment.
Type	PCR SSR
PCR primers	Forward TTAAAAAcAcgcggATcTTcTc at 169 length 22 bases, Tm 62 Reverse gTAcTcAcATATTTcTcggTcT at 264 length 22 bases, Tm 54
SSR size	117 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC20
Source Species	Triticum aestivum