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GrainGenes Probe Report: WMC19

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC19
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1F6 Donor: Nickerson AGR46. CA microsatellite. Faint artefacts found after PCR at 61C and 51C anneal.
Type	PCR SSR
PCR primers	Forward cTgAcATgcggcATTcAcTTcc at 85 length 22 bases, Tm 69 Reverse AggcTTAgAAcAcAccgAcAcg at 216 length 22 bases, Tm
SSR size	153 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 51C anneal, product;
Sequence	WMC19
Source Species	Triticum aestivum