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GrainGenes Probe Report: WMC17

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC17  [ Marker Report ]
Locus	Xwmc17-7B [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1C6 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 182 bases located on 7A AGR43. CA microsatellite. Trimed to Rsa1 fragment. (2 polymorphic loci?).
Type	PCR SSR
PCR primers	Forward AccTgcAAgAAATTAggAAcTc at 3 length 22 bases, Tm 58 Reverse cTAgTgTTTcAAATATgTcggA at 163 length 22 bases, Tm 56
SSR size	181 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC17
Source Species	Triticum aestivum