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GrainGenes Probe Report: WMC13

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC13
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1B5 Donor: Nickerson AGR34. Forward sequence is a composite of 2 sequence runs. 2 CT microsatellites. Expected fragment 109 bases. PCR product obtained (smeary on agrose). Sequence is highly homologous to 1E3 (though not identical). Bases 176-309 (ie 3' side of microsatellite) match part of 9F3.
Type	PCR SSR
PCR primers	Forward gTgTcggATgcgcgcgATAgAAT at 80 length 23 bases, Tm 73 Reverse ATgcATAAAAcgcgAccTccccT at 166 length 23 bases, Tm 70
SSR size	109 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC13
Source Species	Triticum aestivum