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GrainGenes Probe Report: WMC10

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC10  [ Marker Report ]
Locus	Xwmc10a Xwmc10-7B wmc10 wmc10-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1G4 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 214 bases located on 7B, 276 bases located on 7B AGR31. CA microsatellite. Trimmed to Rsa1 fragment. Expected fragment 267 bases. (Near) duplicate of 1D2, 1d12. Polymorphic on agarose after PCR 61C anneal. (2 loci?) - is one of these the same as 1d12. NB forward primer would not perfectly match 1d12 sequence.
Type	PCR SSR
PCR primers	Forward gATccgTTcTgAggTgAgTT at 10 length 20 bases, Tm 59 Reverse ggcAgcAcccTcTATTgTcT at 257 length 20 bases, Tm 61
SSR size	267 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC10
Source Species	Triticum aestivum