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GrainGenes Probe Report: WMC9

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC9  [ Marker Report ]
Locus	Xwmc9-7A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1B4 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 7A, 500 bases located on 4A AGR26. Trimmed to Rsa1 fragment. CA microsatellite. Expect Fragment 168 bases. Polymorphic on agarose. (2 or 3 polymorphic loci detected?). Bases 8-175 ie. 5' side of microsatellite match 5g10, bases 114-220 (ie. 3' side of microsatellite) match 5b12, also 5F2. Bases 156 to 210 show homology to 12d4 bases 80 to 134 (55 in longest run, maxScore = 51).
Type	PCR SSR
PCR primers	Forward AAcTAgTcAAATAgTcgTgTccg at 75 length 23 bases, Tm 59 Reverse gTcAAgTcATcTgAcTTAAcccg at 220 length 23 bases, Tm 62
SSR size	168 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC9
Source Species	Triticum aestivum