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GrainGenes Probe Report: WMC7

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	wmc7  [ Marker Report ]
Locus	wmc7 Xwmc007
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1E3 Donor: Nickerson AGR21. Trimmed to Rsa1 fragment. Multple inserts (2). Complex (C)(CA)(GA) repeat in second fragment. Last insert highly homologous to 1B5. Bases 1 to 32 show homology to 19a6 bases 133 to 164 (32 in longest run, maxScore = 27).
Type	PCR SSR
PCR primers	Forward gcTTggAcTAgcTAgAgTATcAT at 356 length 23 bases, Tm 56 Reverse AcAATcTTcAATTcTgTcgcAcg at 536 length 23 bases, Tm 65
SSR size	203 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC7
Source Species	Triticum aestivum