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GrainGenes Probe Report: WMC6

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC6
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1C3 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 95 bases located on 5D AGR19. Trimmed to Rsa1 fragment. (CA)10 microsatellite.
Type	PCR SSR
PCR primers	Forward cccggTTTATAgATAAAgcgcccA at 130 length 24 bases, Tm 69 Reverse AccTcAggAcgAcTcgTcgAcTcA at 200 length 24 bases, Tm 71
SSR size	94 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC6
Source Species	Triticum aestivum