Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC5

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC5
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1B3 Donor: Nickerson AGR18. Trimmed to Rsa1 fragment. (CT)(CA) compound. Bases 187-290 (ie. 3' side of microsatellite) match part of 9e5. Bases 20-140 ie 5' side of microsatellite match part of 4d6.
Type	PCR SSR
PCR primers	Forward TgTTcTTcTggcATTAgATgggTg at 66 length 24 bases, Tm 66 Reverse cTggTTccTcgTcATcgATcTTgg at 343 length 24 bases, Tm 70
SSR size	302 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product;
Sequence	WMC5
Source Species	Triticum aestivum