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GrainGenes Probe Report: WMC4

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC4
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1H2 Donor: Nickerson AGR16> No obvious overlap between forward and reverse sequences. AG or AC microsatellite. 439 +474. Bright smeary fragment on agarose after PCR 61C anneal.
Type	PCR SSR
PCR primers	Forward TAgAAAgggggTTcggTTggTTgg at 262 length 24 bases, Tm 71 Reverse AgccTcTTgggcTgATggATgATg at 412 length 24 bases, Tm 71
SSR size	174 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear;
Sequence	WMC4
Source Species	Triticum aestivum