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GrainGenes Probe Report: WMC1

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC1  [ Marker Report ]
Locus
Xwmc1-3B
Xwmc1
wmc1-3B
wmc1
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 1B1
Donor: Nickerson
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 158 bases located on 3B
AGRO2. (CT)(CA) compound. (CA)14 334 to 361. Primers redesigned 19.6.97 to avoid linkers (old primers deleted from database 19.6.97). Bases 244 to 334 show homology to 2h1.2 bases 27 to 117 (91 in longest run, maxScore = 67). True duplicate with 2h1.2, 1b1 has priority.
Type
PCR
SSR
PCR primers
Forward AcTgggTgTTTgcTcgTTgA at 230 length 20 bases, Tm 64
Reverse cAATgcTTAAgcgcTcTgTg at 358 length 20 bases, Tm 62
SSR size
148 bases expected, Chinese Spring
Sequence
WMC1
Source Species
Triticum aestivum