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GrainGenes Probe Report: STSLr19-130

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	STSLr19-130  [ Marker Report ]
Locus	Lr19 Sr25
Reference	Prins R et al. (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat Theoretical and Applied Genetics 103:618-624.
Type	STS
STS primers	5' CAT CCT TGG GGA CCT C 3' 5' CCA GCT CGC ATA CAT CCA 3'
Amplification Conditions	4 min at 94 C, 30 cycles of 30 s at 94 C, 30 s at 60 C, 30 s at 72 C and a final extension for 5 min at 72 C.