Query (optional)   in Class  

GrainGenes Probe Report: MWG2200

[Submit comment/correction][What is a probe?]

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
MWG2200  [ Marker Report ]
Locus
MWG2200
Reference
ReferenceMarcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500.
General Remarks
Restriction Enzyme for CAPS marker is Ava I
PCR primers
5' agcacggcctccagcaccac 3'
5' agcacggcctccagcaccac 3'
5' atcctggagtacctcgaagccg 3'
5' atcctggagtacctcgaagccg 3'
STS primers
5' ATCCTGGAGTACCTCGAAGCCG
5' CGATGGACGTGCATCTAAGTCG
STS size
272
Amplification Conditions
5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 58 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence
AJ234832
MWG2200-5
Source Species
Hordeum vulgare