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GrainGenes Probe Report: MWG618

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	MWG618  [ Marker Report ]
Locus	MWG618 MWG618-5H
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500.
Type	Genomic STS
PCR primers	5' gccgctaaaacacatcaaac 3' 5' gccgctaaaacacatcaaac 3' 5' tattttcagtcccgcacac 3' 5' tattttcagtcccgcacac 3'
STS primers	5' TGATTGTATCGGATGATTGC 5' AGCTGAGAAATGAAGAAGGG
STS size	180
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 65 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence	AJ234539 AJ234538 MWG618-5 MWG618-3
Insert Enzyme	PstI
Source Species	Hordeum vulgare