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GrainGenes Probe Report: MWG584

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	MWG584  [ Marker Report ]
Locus	Xmwg584-3 [ Show all 6 ]
Synonym	Synonym HAR35_23373 HAR35_30482
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500. Graner A et al. (1991) Construction of an RFLP map of barley Theoretical and Applied Genetics 83:250-256.
General Remarks	Restriction Enzyme for CAPS marker is Stu I
Type	Genomic STS
PCR primers	5' aaggcaagattggacgaggttt 3' 5' aaggcaagattggacgaggttt 3' 5' ttggaggagagcggaagagat 3' 5' ttggaggagagcggaagagat 3'
STS primers	5' GTATTCTTGGAGGAGAGCGG 5' CGAGGTTTACCCTGGAGACG
STS size	365
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 58 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence	AJ234524 AJ234523 MWG584-5 MWG584-3
Linkage Group	3H
Insert Enzyme	PstI
Source Species	Hordeum vulgare
Insert Size	1.9