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GrainGenes Probe Report: UBW18

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
UBW18
Locus
ubw18
ubw18a
ubw18a-7B
ubw18b
ubw18b-7B
Reference
ReferenceTerracciano I et al. (2013) Development of COS-SNP and HRM markers for high-throughput and reliable haplotype-based detection of Lr14a in durum wheat (Triticum durum Desf.). Theoretical and Applied Genetics 126:1077-1101.
General Remarks
See Table 1 in TAG-126-1077 for allele-specific bases
Anneling temperature = 60
Type
COS-SNP
PCR primers
Forward Creso/Colosseo specific F-C: cacgacgttgtaaaacgaCCTCCTCTCTGATGCAATTGTATGTC
Forward Lloyd specific F-L: CAATTGTGTCTGCTTCATCGTG
R: CCTGGGTTTATTTACTGTCACTAC