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GrainGenes Probe Report: HvXYLISOG

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HvXYLISOG  [ Marker Report ]
Locus	HvXYLISOG
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks	Insert contains repeat (tcc)4 Polymorphic Information Content (PIC) = 0.14 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	Left 5' CGGACCTGGTTTCCTCCTCC Right 5' AGATGGGGTTCTGTCACGGC
SSR size	170
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	X95256
Source Species	Hordeum vulgare