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GrainGenes Probe Report: HvWaxy4

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HvWaxy4  [ Marker Report ]
Locus	HvWaxy4 HvWaxy4b HvWaxy4a HvWaxy
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GCAT)4 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	(GCAT)4GBM1326 PCR_primers (GCAT)4Hv49505 PCR_primers 5' AGTATCGCAGACGCTCAC 3' 5' GTTATGTACTCGCTCGCTC 3' AGTATCGCAGACGCTCAC GTTATGTACTCGCTCGCTC
SSR size	169
Amplification Conditions	SSR_size 1 cycle of 3 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 53C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare