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GrainGenes Probe Report: HvMUB1

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
HvMUB1  [ Marker Report ]
Locus
HvMUB0001
HvMUB1
Reference
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferencePillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks
Insert contains repeat (t)14
Type
SSR
PCR primers
GAAACGAAGCGATGCTGTGAC
AGCAAAAGAATTGGCCTCGC
Left 5' GAAACGAAGCGATGCTGTGAC
Right 5' AGCAAAAGAATTGGCCTCGC
SSR size
128
Amplification Conditions
Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Source Species
Hordeum vulgare