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GrainGenes Probe Report: HVMLOH1A

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HVMLOH1A  [ Marker Report ]
Locus	HVMLOH0001A HVMLOH1A
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GA)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.52
Type	PCR SSR
PCR primers	5' CCTCCCCTCTGATATGATAA 3' 5' GTACAGACGGTTTAATTGTCC 3' CCTCCCCTCTGATATGATAA GTACAGACGGTTTAATTGTCC Forward CCTCCCCTCTGATATGATAA Reverse GTACAGACGGTTTAATTGTCC
SSR size	175
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare