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GrainGenes Probe Report: HVM23

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HVM23  [ Marker Report ]
Locus	HVM23 HVM023
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks	Insert contains repeat (GA)9.
Type	PCR SSR
PCR primers	5' TCGGTGAAGAAATACGAGGC 3' 5' TCTTTGTAGACCTACCGGTCC 3' TCGGTGAAGAAATACGAGGC TCTTTGTAGACCTACCGGTCC
SSR size	0.246
Amplification Conditions	The 'touchdown' PCR comprised 38 cycles of 94oC for 1 min denaturing, and 72oC for 1 min extension. Annealing (30 sec) temperatures were progressively decreased by 1oC every second cycle from 69oC to 60oC, at which temperature they remained constant for the final 20 cycles. The reaction ended with a 5-min extension at 72oC.
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.