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GrainGenes Probe Report: HVM5

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HVM5  [ Marker Report ]
Locus	HVM5 HVM005 HVM05
Reference	Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876.
General Remarks	Pathogenesis-related protein Insert contains repeats (GT)6 and (AT)16.
Type	PCR SSR
PCR primers	5' AACGACGTCGCCACACAC 3' 5' AGGAACGAAGGGAGTATTAAGCAG 3'
SSR size	0.202
Amplification Conditions	PCR comprised one cycle of 94oC for 3 min, 55oC for 2 min, and 72oC for 1.5 min, followed by 30 cycles of 94oC for 1 min, 55oC for 2 min, and 72oC for 1.5 min.
Sequence	X58566
Insert Enzyme	TaqI
Source Species	Hordeum vulgare
Source Germplasm	Old ZZ (barley)
DNA Origin	Size-fractionated genomic library screened with synthetic dinucleotide repeats (CA)10 and (GA)10.