Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: HVCMA

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HVCMA  [ Marker Report ]
Locus	HVCMA M-HVCMA HVCMA-7H
Synonym	Synonym HAR35_14731 HAR35_14732
Reference	Becker J and Heun M (1995) Barley microsatellites: allele variation and mapping Plant Molecular Biology 27:835-845.
General Remarks	PCR size is expected size (kb) based on published sequence. Insert contains repeat (AT)9. GTAAAGCAAATGTTGAGCAACG produced clearer bands than the alternative (overlapping) primer, GAGCAACGGGAGTCTCACATAT.
Type	PCR SSR
PCR primers	5' GCCTCGGTTTGGACATATAAAG 3' 5' GTAAAGCAAATGTTGAGCAACG 3'
SSR size	0.141
Sequence	X59264
Source Gene Class	Inhibitor of alpha-amylase
Source Species	Hordeum vulgare