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GrainGenes Probe Report: HVBAMY

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HVBAMY  [ Marker Report ]
Locus	HVBAMY
Synonym	Synonym HAR35_14441 HAR35_14442 HAR35_27240
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks	Insert contains repeat (a)17 Polymorphic Information Content (PIC) = 0.57 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	CAAGCAAAAGGTTAAAGGTGCC TTGTCGAAACCCATCCGCTC Forward CAAGCAAAAGGTTAAAGGTGCC Reverse TTGTCGAAACCCATCCGCTC Left 5' CAAGCAAAAGGTTAAAGGTGCC Right 5' TTGTCGAAACCCATCCGCTC
SSR size	132
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	D63574
Source Species	Hordeum vulgare