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GrainGenes Probe Report: HvB23D4

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HvB23D4  [ Marker Report ]
Locus	HvB23D4
Synonym	Other name HvOLE1
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
General Remarks	Insert contains repeat(GCCT)4 SSR size deduced from sequence: 198 bp
Type	PCR
PCR primers	5' GATGGATGTCAGTCGGTC 3' ATGAGCAGTAGTACAACTCTAAGC Forward GATGGATGTCAGTCGGTC Reverse ATGAGCAGTAGTACAACTCTAAGC
Amplification Conditions	40 cycles touchdown at 94, 64-55, 72 C
Sequence	X76603
Source Gene Class	Oleosin
Source Species	Hordeum vulgare