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GrainGenes Probe Report: HvACL3

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HvACL3  [ Marker Report ]
Locus	HvACL3
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks	Insert contains repeat (t)10 Polymorphic Information Content (PIC) = 0.36 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	Forward CCGTCTAGTAGGAGGAGTTATGG Reverse CAAATCTTGTTCTCGCGGTCG Left 5' CCGTCTAGTAGGAGGAGTTATGG Right 5' CAAATCTTGTTCTCGCGGTCG
SSR size	204
Amplification Conditions	High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Source Species	Hordeum vulgare