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GrainGenes Probe Report: GPW4043

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GPW4043  [ Marker Report ]
Locus	Xgpw4043-2B Sr47 gpw4043 gpw4043-2B
Reference	Klindworth DL et al. (2012) Introgression and Characterization of a Goatgrass Gene for a High Level of Resistance to Ug99 Stem Rust in Tetraploid Wheat G3: Genes, Genomes, Genetics 2:665-673. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. Sr47 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Motif (GA)16
Type	PCR SSR
PCR primers	Forward ACATATGCACGCACGCAC Reverse CATTGACACCCCTGACACTC 5'- ACATATGCACGCACGCAC -3' 5'- CATTGACACCCCTGACACTC -3'
SSR size	Expected 162
Amplification Conditions	Tm = 60C Denaturing step: 94C, 3 min. Touchdown step 1 (0.5C down each cycle until final annealing temperature): 94C, 30 sec; 65C, 30 sec; 72C, 2 min. Amplification step (30 cycles): 94C, 30 sec; 60C, 30 sec; 72C, 2 min. Extension step: 72C, 10 min
Linkage Group	2B
Source Species	Triticum aestivum