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GrainGenes Probe Report: GMS3

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
GMS3  [ Marker Report ]
Locus
GMS0003
GMS003
GMS3
Reference
ReferenceHayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.
ReferenceHearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
ReferenceStruss D and Plieske J (1998) The use of microsatellite markers for detection of genetic diversity in barley populations. Theoretical and Applied Genetics 97:308-315.
General Remarks
Annealing temperature = 60 deg C
Insert contains repeat (gt)15
Type
SSR
PCR primers
5' TTTCAGCATCACACGAAAGC
5' TTGCATGCATGCATACCC
Forward TTTCAGCATCACACGAAAGC
Reverse TTGCATGCATGCATACCC
TTTCAGCATCACACGAAAGC
TTGCATGCATGCATACCC
SSR size
144