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GrainGenes Probe Report: GBS539

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GBS539  [ Marker Report ]
Locus	GBS0539 GBS0539-5H
Reference	Stein N et al. (2007) A 1000 loci transcript map of the barley genome - new anchoring points for integrative grass genomics Theoretical and Applied Genetics 114:823-839.
General Remarks	Clone HW05H01V
PCR primers	ACAATCGATCCAAGGCGTGA GGACCTCCACCGGTTCGT
Amplification Conditions	5 min at 94 deg C; 50 cycles with 30 sec at 94 deg C, 1 min at 65 deg C (touchdown of 1 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 40 cycles), 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	AL504487
DNA Library	IPK-HW
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)