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GrainGenes Probe Report: GBM1509

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	GBM1509  [ Marker Report ]
Locus	GBM1509 GBM1509-4H
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2006) Genetic mapping and BAC assignment of EST-derived SSR markers shows non-uniform distribution of genes in the barley genome Theoretical and Applied Genetics 113:239-250.
General Remarks	Primer sequences for this SSR to be released in the public domain soon (noted: September 2006). Those interested should contact Andreas Graner Clone HU10P22r
Type	SSR EST
PCR primers	CAATCGTTGTCCAGAACCCT GGCCGACAAATATGCTTCAT Forward CAATCGTTGTCCAGAACCCT Reverse GGCCGACAAATATGCTTCAT
Amplification Conditions	3 min at 94 deg C; 45 cycles with 30 sec at 94 deg C, 30 sec at 60 deg C (touchdown of 0.5 deg C / cycle for initial 10 cycles - final annealing of 55 deg C for remaining 35 cycles), 30 sec at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	CA008447
DNA Library	IPK-HU
Source Species	Hordeum vulgare
Source Germplasm	Barke (barley)