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GrainGenes Probe Report: EBmag757

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
EBmag757  [ Marker Report ]
Locus
EBmag0757
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AG)31
Quality = 2: Single product, relatively strong stutter bands
Diversity Index (DI) = 0.68
Type
PCR
SSR
PCR primers
5' GTGTCTTTTTCACTTCCTTTG 3'
5' TCTTCACTGTTGAGATGATGA 3'
SSR size
116
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare