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GrainGenes Probe Report: EBmac691

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
EBmac691  [ Marker Report ]
Locus
EBmac0691
EBmac691
Synonym
Other nameEBmac0691
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CA)3CG(CA)7
Quality = 1: Single product, only weak stutter bands
Type
PCR
SSR
PCR primers
5' TTAACAGAGGGCATTGGT 3'
5' TCCTTTTCTCCATTTGAGTT 3'
SSR size
111
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C, 2.5 nM of MgCl2
Source Species
Hordeum vulgare