GrainGenes Probe Report: EBmac684
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
EBmac684 [ Marker Report ]
Locus
EBmac0684 EBmac684 EBmac0684b
Synonym
Other name Bmac684 Ebmac0684 EBmac0684
Reference
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (TA)7(TG)11, (TG)11(TTTG)5 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.64
Type
PCR SSR
PCR primers
5' TTCCGTTGAGCTTTCATACAC 3'
5' ATTGAATCCCAACAGACACAA 3'
SSR size
172
Amplification Conditions
1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Probe Report: EBmac684
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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