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GrainGenes Probe Report: EBmac659

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
EBmac659  [ Marker Report ]
Locus
EBmac0659
EBmac659
Synonym
Other nameEBmac0659
Reference
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (AC)10AT(AC)7
Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type
PCR
SSR
PCR primers
5' ACCTAGCAAAGTCCAAAGTATCC 3'
5' GAGAGAGGTTGTACGACTGAGG 3'
SSR size
154
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare