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GrainGenes Probe Report: EBmac603

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
EBmac603  [ Marker Report ]
Locus
EBmac0603
EBmac603
Synonym
Other nameEBmac0603
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
ReferenceRamsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (CA)10
Quality = 1: Single product, only weak stutter bands
Diversity Index (DI) = 0.74
Type
PCR
SSR
PCR primers
5' ACCGAAACTAAATGAACTACTTCG 3'
5' TGCAAACTGTGCTATTAAGGG 3'
ACCGAAACTAAATGAACTACTTCG
TGCAAACTGTGCTATTAAGGG
SSR size
149
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare