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GrainGenes Probe Report: CFD251

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	CFD251  [ Marker Report ]
Locus	Xcfd251-1B cfd251 cfd251-2A
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CCTT)3 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GAACATGAAGACGAGGAGGC 3' Reverse 5' AAAGCATCTTTGCTTCTCGC 3'
SSR size	169
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	1B
Source Species	Aegilops tauschii