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GrainGenes Probe Report: CFD71

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	CFD71  [ Marker Report ]
Locus	Xcfd71-4D [ Show all 8 ]
Reference	Hiebert CW et al. (2010) An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67) Theoretical and Applied Genetics 121:1083-1091. Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172. Hiebert CW MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Leaf Rust Resistance. Lr67 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)10(GA)30 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CAATAAGTAGGCCGGGACAA 3' Reverse 5' TGTGCCAGTTGAGTTTGCTC 3'
SSR size	216
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4D/4A
Source Species	Aegilops tauschii