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GrainGenes Probe Report: CFA2256

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	CFA2256  [ Marker Report ]
Locus	Xcfa2256-4A cfa2256 cfa2256-4A
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TG)29 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' GGTAATATTCAGGTTACCGCACA 3' Reverse 5' GGTAAAGTTATAAATTGTTGTGGGC 3'
SSR size	171
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4A
Source Species	Triticum urartu