GrainGenes Probe Report: CFA2173
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
CFA2173 [ Marker Report ]
Locus
Xcfa2173-4A Xcfa2173-4D Xcfa2173 Xcfa2173b Xcfa2173-6A
Reference
Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)28 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' GACATACTCCGGCGTTGAAT 3'
Reverse 5' TTCCCAGGACATCCTTCTTG 3'
SSR size
258
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
?/4D
Source Species
Triticum urartu
GrainGenes Probe Report: CFA2173
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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