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GrainGenes Probe Report: CFA2091

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	CFA2091  [ Marker Report ]
Locus	Xcfa2091-4B Xcfa2091 cfa2091 cfa2091-4B
Reference	Sourdille P et al. (2001) Improvement of the genetic maps of wheat using new microsatellite markers Plant & Animal Genome IX Abstracts 167.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CTT)4(TCC)5 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' CCTTCTTAATGACCGGCTCG 3' Reverse 5' AAACGAAGACGTGGACATGG 3'
SSR size	191
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	4B
Source Species	Triticum urartu