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GrainGenes Probe Report: AM59

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM59
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (G)12..(GA)5 Known repeat = Repetitive DNA with 87 % identity over 31 base pairs
Type	PCR SSR
PCR primers	5'TCT GGG CTA CGA AAA TGT T 5'CCG GCG GAA ATG GAG TG
SSR size	275
Amplification Conditions	Average melting temperature (degrees C) = 44
Source Species	Avena sativa
Source Germplasm	Ogle (oat)