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GrainGenes Probe Report: AM44

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM44
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (GAA)11 Known repeat = Retrotransposon with 76 % identity over 43 base pairs Among 20 oat cultivars, 1 allele was found.
Type	PCR SSR
PCR primers	5'CGT TGG CCC CTT TTT TCA GTG 5'AGG GGC ATT GGC TTT GTC C
SSR size	174
Amplification Conditions	Average melting temperature (degrees C) = 49
Source Species	Avena sativa
Source Germplasm	Ogle (oat)