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GrainGenes Probe Report: AM42

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
AM42  [ Marker Report ]
Locus
am42
AM42
am42a
am42b
Reference
ReferenceFu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038.
ReferenceLi CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks
Insert contains repeat (GAA)16
Polymorphic in Kanota/Ogle but not inMarion/Terra.
Among 20 oat cultivars, 3 alleles were found, polymorphic information content (PIC) = 0.59.
In 369 Avena sterilis accessions there were 6 alleles of size 188-201 bp, PIC = 0.4.
Type
Genomic
PCR
SSR
PCR primers
5'GCT TCC CGC AAA TCA TCA T
5'GAG TAA GCA AAG GCC AAA AAG T
SSR size
193
Amplification Conditions
Average melting temperature (degrees C) = 45
Source Species
Avena sativa
Source Germplasm
Ogle (oat)