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GrainGenes Probe Report: AM30

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM30
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (GAA)14 Polymorphic in Marion/Terra but not in Kanota/Ogle. Among 20 oat cultivars, alleles at two loci were found. There were 3 alleles at locus AM30a, polymorphic information content (PIC) = 0.65, and 2 alleles at AM30b, PIC = 0.46.
Type	PCR SSR
PCR primers	5'TGA AGA TAG CCA TGA GGA AC 5'GTG CAA ATT GAG TTT CAC G
SSR size	203
Amplification Conditions	Average melting temperature (degrees C) = 43
Source Species	Avena sativa
Source Germplasm	Ogle (oat)