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GrainGenes Probe Report: AM28

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM28
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (GAA)8 Known repeat = L1 repeat with 73 % identity over 49 base pairs Among 20 oat cultivars, 1 allele was found.
Type	PCR SSR
PCR primers	5'GAC CTC TTG AGT AAG CAA CG 5'TGG TCT TCC TAT CCA CAA TG
SSR size	135
Amplification Conditions	Average melting temperature (degrees C) = 46
Source Species	Avena sativa
Source Germplasm	Ogle (oat)