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GrainGenes Probe Report: AM25

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM25
Locus	am25
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AC)8..(AC)4(CT)4 Known repeat = Satellite DNA with 61 % identity over 88 base pairs Among 20 oat cultivars, 3 alleles were found, polymorphic information content (PIC) = 0.64. In 369 Avena sterilis accessions there were 3 alleles of size 220-240 bp, PIC = 0.1.
Type	Genomic PCR SSR
PCR primers	5'AGC CTG GAC ATG TAA TCT GGT 5'AGC CCT GGT CTT CTT CAA CA
SSR size	229
Amplification Conditions	Average melting temperature (degrees C) = 47
Source Species	Avena sativa
Source Germplasm	Ogle (oat)