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GrainGenes Probe Report: S2269949

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	S2269949
Locus	Fr-A2 S2269949
Reference	Sieber AN et al. (2016) Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat Theoretical and Applied Genetics 129:1087-1097.
Type	KASP
PCR primers	5'-FAM-tail-ACTGCAGCGAG-GATTCCCA-3' 5'-AGCCACCTCTGAGATGCCGCTT-3' 5'-HEX-tail- CTACTGCAGCGAGGATTC-CCG-3' 5'-AGCCACCTCTGAGATGCCGCTT-3'
Specificity	FAM-tail primer is specific for the frost susceptible allele of Fr-A2, while the HEX-tailed allele is specific to the frost tolerant allele