Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: AM23

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	AM23
Reference	Li CD et al. (2000) The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars Theoretical and Applied Genetics 101:1259-1268.
General Remarks	Insert contains repeat (AC)19 Known repeat = Retrotransposon with 77 % identity over 45 base pairs Among 20 oat cultivars, 2 alleles were found, polymorphic information content (PIC) = 0.18.
Type	PCR SSR
PCR primers	5'TCT TTA AGG ATT TGG GTG GAG 5'AAT CTT CGA GGG TGA GTT TCT
SSR size	247
Amplification Conditions	Average melting temperature (degrees C) = 45
Source Species	Avena sativa
Source Germplasm	Ogle (oat)